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19 the sequences at the cloning site of three vectors are given below. the bamhi (ggatcc) and hindlll (aagctt) sites are underlined. only the sequences around t
19 The sequences at the cloning site of three vectors are given below. The BamHI (GGATCC) and Hindlll (AAGCTT) sites are underlined. Only the sequences around the restriction sites are shown. The symbol"_ the sequences around indi- cates rest of the sequence. Vector 1: Promoter..ATGGGTCGCGGATC- CGGCTGC. AAGCTT Vector 2: Promote..ATGGGTCGGGATCC- GGCTGCT.. AAGCTT- Vector 3: Promoter..ATGGGTCGGATCC- GGCTGCTA.. AAGCTT Which one of the above three vectors is appropriate to clone the following ORF? ATGCCCAACACCCGGATCC- CG..TAAAAGCTT for expression? Give the reason in one sentence
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Anup Padwal![best-answer]()
All the three can be used as vector, because all have same restriction site for both restriction enzyme.
please explain.... answer is given vector3
if appropriate anser is asked then you can give 3, because it contain less GC than other 2.
can u explain more
yes, see the sequence of bases of vector all contains the cut site for both the restriction enzyme. Also all have sufficient space of base pair atter promoter region. so all vector can use to insert ORF of gene of interest. but if you have to select only one option search for ST rich region after promoter which results in easy sepetration of base pair.