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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Farha Jahan posted an Question
July 31, 2021 • 22:50 pm
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30 points
30 points
- CSIR NET
- Life Sciences
54] the following disulfide bond containing peptide was digested using trypsin. how many peptide fragments will be produced by digestion? mtpqkavilnsctyrpypp 1
54] The following disulfide bond containing peptide was digested using trypsin. How many peptide fragments will be produced by digestion? MTPQKAVILNSCTYRPYPP 1 Three 2 Four 3 Five 4 Six
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Krishan k jakhad Best Answer
A. 3 fragments may generate....as ,trypsin predominantly cleaves proteins at the carboxyl side (or "C-terminal side") of the amino acids lysine and arginine except when either is bound to a C-terminal proline although large-scale mass spectrometry data suggest cleavage occurs even with proline.Trypsin is considered an endopeptidase, i.e., the cleavage occurs within the polypeptide chain rather than at the terminal amino acids located at the ends of polypeptides.