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A DNA segment was cloned into the active site region of lacZ gene and the recombinant plasmid introduced into lacZ- strain
A DNA segment was cloned into the active site region of lacZ gene and the recombinant plasmid introduced into lacZ- strain of E.coli and plated on a medium containing X-gal. The colonies showed blue colour. Which one of the following statements is true ?
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Eduncle Best Answer
Blue color of colonies is due to beta galactosidase activity which shows lacZ gene expression. It was observed that the insertion of DNA segment did not impact the activity of lacZ gene expression and shows blue color colonies. Possible reason for this observation is that inserted DNA segment could be a group1 intron which posses self spliced property so it get removed during processing and it will not be a part of mature DNA.