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Eduncle posted an MCQ
December 28, 2019 • 20:01 pm 0 points
  • CSIR NET
  • Life Sciences

A group of scientists performed an experiment where they artificially fused mouse cells with monkey cells. The resulting fused cells were labelled with fluorescently tagged antibodies against mouse and monkey surface receptor proteins, X and Y respectively. At the time 0 minute just after fusion events, two receptors were confined to their own half in the heterokaryon. However, such surface receptors (X and Y) intermixed on the cell surface after 60 minutes. Which one of the given statements correctly reflects the outcome of the experiment ?

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    Eduncle Best Answer

    The fluorescent tags are attached to surface receptors X and Y, on mouse and monkey cells respectively. After fusion both the tags remain on the surface and are not lost with time. Only with time they change their position. This shows that membrane proteins are not static but dynamic. Since the tags are not lost with time so, they surface proteins are not exchanged with the cytoplasmic protein. Since the tags are specific so for surface proteins so no conclusion about the cytoplasmic proteins can be drawn for this experiment.

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