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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Abhilasha Ganesh Tak posted an Question
July 07, 2021 • 11:40 am
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30 points
30 points
- CSIR NET
- Life Sciences
(a) here in second strand synthesis why is the dna pol 4dntps are used after cutting with sal1
(a) -AAA, 3 mRNA Oligo-dT AAAn TTTn 5 () Reverse transcriptase + 4dNTPs (2) Alkali First strand synthesis -TTT,S' 3 DNA polymerase (Klenow) + 4dNTPs Second strand synthesis Sall-linkers, ligation s ss (1) Nuclease S1 (2) DNA polymerase (Klenow) + 4dNTPs EcORI-linkers, ligation RR s SsR R Cut with Sall, EcoRI Insert into vector cut with Sall and EcORI (c)
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Krishan k jakhad Best Answer
just to make free ends so that new dNTPs can be incorporated when nuclease further degrade. Sal I is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.