Abhilasha ganesh tak Asked a Question
July 7, 2021 6:10 ampts 30 pts
(a) -AAA, 3 mRNA Oligo-dT AAAn TTTn 5 () Reverse transcriptase + 4dNTPs (2) Alkali First strand synthesis -TTT,S' 3 DNA polymerase (Klenow) + 4dNTPs Second strand synthesis Sall-linkers, ligation s ss (1) Nuclease S1 (2) DNA polymerase (Klenow) + 4dNTPs EcORI-linkers, ligation RR s SsR R Cut with Sall, EcoRI Insert into vector cut with Sall and EcORI (c)
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  • Krishan k jakhad Best Answer
    just to make free ends so that new dNTPs can be incorporated when nuclease further degrade. Sal I is a restriction endonuclease used for molecular biology methods to cleave DNA at ...
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