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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Mohammad Aimr posted an Question
October 07, 2020 • 13:36 pm
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30 points
30 points
- CSIR NET
- Life Sciences
A mutant of e. coli with a heat sensitive dna iigase (25°c permissive, 37°c non-permissive) has been used to show that dna synthesis is discontinuous. examinati
A mutant of E. coli with a heat sensitive DNA Iigase (25°C permissive, 37°C non-permissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of H]-Thymidine in the mutant would demonstrate the accumulation of short segments of a. unlabeled DNA at 25°C and at 37°C b. unlabeled DNA at 25°C but not at 37°C C. radioactive DNA at 37°C but not at 25°C d. radioactive DNA at 25°C but not at 37°c
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Anup Padwal![best-answer]()
Option D Because DNA replication process complete when ligase is active. unless no replication. option A and B are wrong. because at 25*C replication ligase active and complete replication. Option C wrong because no replication process due to inactive ligase.