A mutant of e. coli with a heat sensitive dna iigase (25°c permissive, 37°c non-permissive) has been used to show that dna synthesis is discontinuous. examinati

A mutant of E. coli with a heat sensitive DNA Iigase (25°C permissive, 37°C non-permissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of H]-Thymidine in the mutant would demonstrate the accumulation of short segments of a. unlabeled DNA at 25°C and at 37°C b. unlabeled DNA at 25°C but not at 37°C C. radioactive DNA at 37°C but not at 25°C d. radioactive DNA at 25°C but not at 37°c