A plasmid with a linking number (Lk) of 200, topological winding (Tw) number of 200 and a writhing number (Wr) of 0 was transformed into E coli. The plasmid was re-isolated from the culture of the transformant. The re-isolated plasmid was found to possess the same molecular weight as the original plasmid, but it possessed a writing number of –5. Following statements are made about this observation.
(A)    Lk of the re-isolated plasmid would be 195.
(B)    Lk of the re-isolated plasmid would remain 200.
(C)    Tw of the re-isolated plasmid would remain 200.
(D)    Tw of the re-isolated plasmid would be 195.
Which one of the following combinations of the above statements is the correct representation of the facts.

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