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A plasmid with a linking number (Lk) of 200, topological winding (Tw) number of 200 and a writhing number (Wr) of 0 was transformed into E coli. The plasmid was re-isolated from the culture of the transformant. The re-isolated plasmid was found to possess the same molecular weight as the original plasmid, but it possessed a writing number of –5. Following statements are made about this observation.
(A) Lk of the re-isolated plasmid would be 195.
(B) Lk of the re-isolated plasmid would remain 200.
(C) Tw of the re-isolated plasmid would remain 200.
(D) Tw of the re-isolated plasmid would be 195.
Which one of the following combinations of the above statements is the correct representation of the facts.
Linking number = Twist + Writhe. Linking number of the structure does not change. Since L is constant in an intact duplex DNA circle, for every new double helical twist,deltaT, ...