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An integral membrane protein (P) has been identified as a cell surface protein of hepatocytes and assigned to bind to hepatitis B virus (HBV) and promote its entry into cytosol. Upon binding to HBV particles, the C-terminal of P interacts with F-actin in the cytosol and in turn, helps in the entry of the HBV particles. P was successfully cloned and expressed in animal cells in culture where in its N-terminal is exposed on the surface while the C-terminal resides in the cytosol. The recombinant protein P so expressed retains its complete structure and function. From the list of experiments given below, which one of the experiments will you perform to show the C-terminal of the protein P via interacting with F-actin helps in HBV entry ?
In host pathogen interaction the C terminal of HBV(P) protein interacts with F actin of host and viral entry occurs. To confirm the importance of C terminal part of (P) protein,...