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Eduncle Best Answer
In host pathogen interaction the C terminal of HBV(P) protein interacts with F actin of host and viral entry occurs. To confirm the importance of C terminal part of (P) protein, Wild type and mutant type (P) protein (defective for C terminal end) can be compared. The protein protein interaction can be studied using immunoprecipitation and blotting. This can be done by incubating radiolabeled HBV(P) along with F actin and F actin autoantibodies. The (P) with C part will interact with radiolabeled HBV and can be detected. The defective (P) will not interact rather F actin and antibodies for F actin will interact.