An integral membrane protein (P) has been identified as a cell surface protein of

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December 28, 2019 2:17 pm

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AIncubating radiolabelled HBV with hepatocytes in culture and follow up its association with F-actin by immuno-precipitation analysis using anti-F-actin antibody.

BIncubating radiolabelled HBV with hepatocytes over-expressing the C-terminal mutant of P and repeat the rest of the experiment as in

CIncubating radiolabelled HBV with hepatocytes over expressing the N-terminal mutant of P and repeat the rest of the experiment as in

DUsing wild type P as well as C-terminal mutant of P and their individual over expression in a heterologous cell line (completely devoid of endogenous P protein) and then repeat experiment as in

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In host pathogen interaction the C terminal of HBV(P) protein interacts with F actin of host and viral entry occurs. To confirm the importance of C terminal part of (P) protein,...
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