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Eduncle posted an MCQ
December 28, 2019 • 19:47 pm 0 points
  • CSIR NET
  • Life Sciences

An integral membrane protein (P) has been identified as a cell surface protein of hepatocytes and assigned to bind to hepatitis B virus (HBV) and promote its entry into cytosol. Upon binding to HBV particles, the C-terminal of P interacts with F-actin in the cytosol and in turn, helps in the entry of the HBV particles. P was successfully cloned and expressed in animal cells in culture where in its N-terminal is exposed on the surface while the C-terminal resides in the cytosol. The recombinant protein P so expressed retains its complete structure and function. From the list of experiments given below, which one of the experiments will you perform to show the C-terminal of the protein P via interacting with F-actin helps in HBV entry ?

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    Eduncle Best Answer

    In host pathogen interaction the C terminal of HBV(P) protein interacts with F actin of host and viral entry occurs. To confirm the importance of C terminal part of (P) protein, Wild type and mutant type (P) protein (defective for C terminal end) can be compared. The protein protein interaction can be studied using immunoprecipitation and blotting. This can be done by incubating radiolabeled HBV(P) along with F actin and F actin autoantibodies. The (P) with C part will interact with radiolabeled HBV and can be detected. The defective (P) will not interact rather F actin and antibodies for F actin will interact.

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