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we can't calculate protein stability with given information of number of Amino acids only. The hydrogen bonds make this structure especially stable. therefore require complete sequence information. or Methods of Determining Protein Stability Some of the Most Common Methods Used:i Dfferential Scanning Calorimetry (DSC) , Pulse-Chase Method. Bleach-chase method. Cycloheximide-chase method. Circular Dichroism (CD) Spectroscopy. Fluorescence-based Activity Assays.