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Cll 64. an enzymatic reaction exhibits michaelis-menten kinetics. for this reaction, on doubling the concentration of enzyme while maintaining [s] >>e]. a) both
CLL 64. An enzymatic reaction exhibits Michaelis-Menten kinetics. For this reaction, on doubling the concentration of enzyme while maintaining [S] >>E]. a) Both Km and Vmax Will remain the same b) K Will remain the same but Vmax Will increase. c)Km will increase but Vmax Will remain the sanme. c) d) Both Km and Vmax will increase. GATE 20171
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Krishan k jakhad![best-answer]()
B. since, Vmax = Kcat x Enzyme concentration Km = substrate concentration when velocity is half the Vmax. Km is a constant for a given substrate acting on a given enzyme. However, Vmax is directedly proportional to enzyme concentration as Kcat is a constant for a given enzyme. This is very well possible that for a pair of given substrate and given enzyme (with variable enzyme concentration), that Vmax is variable and Km is always a constant. here we are increasing 2x enzyme concentration so rate of reaction should double and approaches Vmax.