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During translation in prokaryotes, when ribosomes reach the termination codon, the termination codon is recognized by the class I release factors (RF1 and RF2) leading to the release of the polypeptide. A second class II release factor (RF3) facilitates the termination process. Which of the following statements regarding the mechanism of action of the release factors is INCORRECT ?
Free RF3 binds GDP very strongly, and the spontaneous dissociation of the guanine nucleotide is slow, implying that cytoplasmic RF3 is in the GDP, rather than the GTP, conformat...