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Explain muller hinton agar for antibiotic testing with explain
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Mueller–Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing, specifically disk diffusion tests. It is also used to isolate and maintain Neisseria and Moraxella species. It typically contains: 2.0 g beef extract. 17.5 g casein hydrolysate. Mueller and Hinton developed Mueller Hinton Agar (MHA) in 1941 for the isolation of pathogenic Neisseria species. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique. Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species. This type is also commonly used for susceptibility testing of Campylobacter. Composition of MHA IngredientsIn Gram/LitreBeef Extract2.00 gmAcid Hydrolysate of Casein17.50 gmStarch1.50 gmAgar17.00 gmDistilled Water1000 ml Final pH 7.3 ± 0.1 at 25ºC