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Priya sarda
Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome. In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies. ... This mechanism is conserved from prokaryotes to eukaryotes and is known as semiconservative DNA replication. Features of Eukaryotic DNA Replication DNA replication uses a semi-conservative method that results in a double-stranded DNA with one parental strand and a new daughter strand. It occurs only in the S phase and at many chromosomal origins. Takes place in the cell nucleus. Synthesis occurs only in the 5′to 3′direction. THE ENZYMES OF DNA REPLICATION Helicases: Unwind the DNA helix at the start of replication. SSB proteins: Bind to the single strands of unwound DNA to prevent reformation of the DNA helix during replication.