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Eduncle Best Answer
Option C and D are correct. Surface Plasmon resonance has association/dissociation constants ranging even from nM to fM. So, option A is wrong.
In proteomics, de novo sequencing is the process of deriving peptide sequences from tandem mass spectra without the assistance of a sequence database. So, B is again wrong.
Hydrogen atoms can be located accurately and precisely by x-ray crystallography.
CD gives information about secondary and tertiary conformations of the molecule. However, it does not give the residue-specific information that can be obtained by X-ray crystallography or NMR.