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Hemolysis of of human blood with anticoagulant edta ,centrifuge in 5 tubes with 0.5 ml of nacl solution of 0.9%,0.6%,0.5%,0.4%,0.3% what will hsppen to each tu
hemolysis of of human blood with anticoagulant EDTA ,centrifuge in 5 tubes with 0.5 ml of nacl solution of 0.9%,0.6%,0.5%,0.4%,0.3% what will hsppen to each tubes
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Hypotonic solution with 0.5 percent NaCl causes RBC to expand and rupture owing to the difference in osmotic pressure. The erythrocyte shrinks in hypertonic solutions and swells in hypotonic solutions. ... The red blood cell has its normal volume in isotonic NaCl. Erythrocytes remain intact in NaCl 0.9%, resulting in an opaque suspension. The RBCs will therefore, shrink. However, reverse will take place in case these are kept in contact with 0.6% NaCI solution which has less osmotic pressure. Water will now move into the RBCs and they will swell. If red blood cells are placed in a 0.3 M NaCl solution, there is little net osmotic movement of water, the size and shape of the cells stay the same; the NaCl solution is isotonic to the cell. when the blood cells are placed in 0.4% sodium chloride solution, water flows into the cells and the cells swell.