A. Kirti posted an Question
January 12, 2021 • 19:57 pm 30 points
  • CSIR NET
  • Life Sciences

How can i find which type of inhibitors are there based on the given data

following data are obtained for an enzyme-catalyzed reaction in the presence and absence of inhibitor A V(mmol ml min) [S](mM) Without A With A 0.2 5.0 3.0 0.4 7.5 5.0 0.8 10.0 7.5 1.0 10.7 8.3 2.0 12.5 10.7 4.0 13.6 12.5 1, Using double-reciprocal plots of the data, determine the type of inhibition that has occurred. 2. Does inhibitor A combine with E, with ES or with both? Explain. Solution 1. The double-reciprocal plots intersect on the y-axis, so the inhibition is competitive. 2. The inhibitor combined only with E, the free enzyme. A competitive inhibitor cannot combine with ES because inhibitor and the substrate compete for the same binding site on the enzyme.

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    Dr. nitin wahi Best Answer

    1.) Competitive inhibition 2.) During a competitive inhibition the inhibitor competes with the [S] molecules for the same active site and Donot complete for [ES] complex

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