30 points
>
Time management is very much important in IIT JAM. The eduncle test series for IIT JAM Mathematical Statistics helped me a lot in this portion. I am very thankful to the test series I bought from eduncle.
Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- CSIR NET
- Life Sciences
How can i find which type of inhibitors are there based on the given data
following data are obtained for an enzyme-catalyzed reaction in the presence and absence of inhibitor A V(mmol ml min) [S](mM) Without A With A 0.2 5.0 3.0 0.4 7.5 5.0 0.8 10.0 7.5 1.0 10.7 8.3 2.0 12.5 10.7 4.0 13.6 12.5 1, Using double-reciprocal plots of the data, determine the type of inhibition that has occurred. 2. Does inhibitor A combine with E, with ES or with both? Explain. Solution 1. The double-reciprocal plots intersect on the y-axis, so the inhibition is competitive. 2. The inhibitor combined only with E, the free enzyme. A competitive inhibitor cannot combine with ES because inhibitor and the substrate compete for the same binding site on the enzyme.
1 Answer(s)
Answer Now
- 0 Likes
- 1 Comments
- 0 Shares
-
![comment-profile-img]()
>
Do You Want Better RANK in Your Exam?
Start Your Preparations with Eduncle’s FREE Study Material
- Updated Syllabus, Paper Pattern & Full Exam Details
- Sample Theory of Most Important Topic
- Model Test Paper with Detailed Solutions
- Last 5 Years Question Papers & Answers
Sign Up to Download FREE Study Material Worth Rs. 500/-
Dr. nitin wahi Best Answer
1.) Competitive inhibition 2.) During a competitive inhibition the inhibitor competes with the [S] molecules for the same active site and Donot complete for [ES] complex