A. Kirti posted an Question
April 09, 2021 • 01:54 am 30 points
  • CSIR NET
  • Life Sciences

How can we determine the order of transfer of loci on the chromosome

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    Krishan k jakhad best-answer

    interrupted mating experiment: Recall that spontaneous breakage creates a natural gradient of transfer, which makes it less and less likely for a recipient to receive later and later markers. Because we have selected for the earliest marker in this cross, the frequency of recombinants is a function of the order of entry for each marker. Therefore, we can immediately determine the order of the genetic markers simply by looking at the percentage of recombinants. The complete order is leu, thr, ile, mal, trp for example. 3. A cross is made between an Hfr that is met+ thr+ ile+ and an F− that is met− thr- ile-. Interrupted-mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements. These recombinants are tested for the presence.

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