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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- CSIR NET
- Life Sciences
How can we filter microbes present in animal cell culture when our cell size is larger than that of the microbes?
Media for animal cell culture cannot be sterilized by steam because they contain a lots of heat labile components therefore they are sterilized by filtration method which include membrane filters which with variable pore sizes (0.1-0.4 pum) for removal of bacterial, fungal, micro plasma and viral contaminants. and even if we filter the cells from the small pore size then we have to destruct the animal cells to filter.
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Alka pal
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Just think about the tea strainer ...it filters the large sized tea leaves or granules with the water n milk content in a similar way when cell culture is filtered all the microbes are filtered out with the liquid content and one gets pure cells just like tea particles are only left in the strainer whereas the liquid and sugar particles are separated out
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do u mean that animal cells remain in strainer and the microbes are filtered out of it
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yeah it's possible if the small microbes get filtered out from the culture than that of animal cells
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yes i meant that
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Sonampreet kaur
animal cells remains intact if we choose filter size carefully .. as cell has bigger size ... so it will bw intact ..