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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Abhilasha Ganesh Tak posted an Question
April 08, 2021 • 11:53 am
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30 points
30 points
- CSIR NET
- Life Sciences
How the peptides ion are isolated via mass to charge ratio value ?
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Krishan k jakhad Best Answer
In 1913, Thomson measured the mass-to-charge ratio of ions with an instrument he called a parabola spectrograph. Today's mass spectrometer. Typically, mass spectrometers measure the mass-to-charge ratio (m/z) of ionized molecules in the gas phase, which can fly with different rates or circulate with different frequencies under electric and/or magnetic fields in the mass analyzer. Proteins and peptides are nonvolatile and polar compounds, therefore requiring special methods to be transferred into the gas phase without extensive degradation. The most common ionization method for histone protein analysis is electrospray ionization (ESI), where a high voltage is used to create an electrically charged spray (electrospray) that triggers the desolvation of peptides/proteins from solvent droplets into the gas phase. ESI sources are typically combined with RP-HPLC systems, which allow the separation of very complex peptide mixtures prior to MS analysis (LC-MS). LC-MS analysis of peptides and proteins provides information on the molecular weight and the elemental composition of the analyte, if sufficient mass resolution is achieved. In addition, further fragmentation of the ions (precursor ions) in tandem mass spectrometry (MS/MS) enables extrapolating the primary sequence of the peptide of interest and information on the presence and position of PTMs.
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please explain the mechanisn of electrospray
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Electrospray Ionization (ESI) is a method by which solutes present in a solution can be transferred into the gas phase as ions. In that case the analyte is charged by association with one or more of the ions present in the solution. This charging process is part of the electrospray mechanism.
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Krishan k jakhad![best-answer]()
Electrospray Ionization (ESI) is a method by which solutes present in a solution can be transferred into the gas phase as ions. In that case the analyte is charged by association with one or more of the ions present in the solution. This charging process is part of the electrospray mechanism.
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