Inushaa posted an Question
March 14, 2022 • 18:36 pm 30 points
  • CSIR NET
  • Life Sciences

How to calculate ,luria broth,nutrient agar,agar agar ,for certain solution kindly tell me basics for calculation

how to calculate ,luria broth,nutrient agar,agar agar ,for certain solution kindly tell me basics for calculation

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    Krishan k jakhad best-answer

    Broth, also known as, LB medium, Lysogeny broth, Luria broth, or Luria-Bertani medium, is a commonly used nutritionally rich medium for culturing bacteria. First described in 1951 by Giuseppe Bertani, a 1-liter medium i.e. distilled water consists of 10 grams of tryptone, 5 grams of yeast extract, and 10 grams of sodium chloride. If you add Agar 1.5-2% the broth will solidify and you may make plates LA to grow Bacterial colonies.

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  • Priya sarda

    Suspend 28 g of nutrient agar powder in 1 litre of distilled water.

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