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Priya sarda
The deoxyribose in DNA in the presence of acid forms β-hydroxylevulinaldehyde which reacts with diphenylamine to give a blue colour with a sharp absorption maximum at 595nm. In DNA, only the deoxyribose of the purine nucleotides react, so that the value obtained represents half of the total deoxyribose present. AIM: To estimate the amount of DNA present in the given unknown solution by diphenylamine method. PRINCIPLE: When DNA is treated with diphenylamine under the acidic condition a bluish green colored complex is formed which has an absorption peak at 595nm.