Inushaa posted an Question
May 12, 2022 • 23:39 pm 30 points
  • CSIR NET
  • Life Sciences

Importance and significance of estimation of dna by diphenylamine method

importance and significance of estimation of DNA by diphenylamine method

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  • Priya sarda best-answer

    The deoxyribose in DNA in the presence of acid forms β-hydroxylevulinaldehyde which reacts with diphenylamine to give a blue colour with a sharp absorption maximum at 595nm. In DNA, only the deoxyribose of the purine nucleotides react, so that the value obtained represents half of the total deoxyribose present. AIM: To estimate the amount of DNA present in the given unknown solution by diphenylamine method. PRINCIPLE: When DNA is treated with diphenylamine under the acidic condition a bluish green colored complex is formed which has an absorption peak at 595nm.

    dna_estimation_by_dpa_method.pdf
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