Ranjan Sahoo Asked a Question
May 13, 2021 1:29 pmpts 30 pts
In an experiment, a 1 kb fragıhent vith a single BamHI site (as shown below in fígure 'A') is to be cloned in the Smai (CCC GGG) site of a cloning vector of 3kb length (figure 'B'). None of the other enzymes of the multiple cloning site are present in the fragment to be cloned. (A) (B) ILJ 3 0.2 0.8 Based on the information given above, a series of digestions were set up for the potential clones and their fragment profiles are given below: A. BamHI 200bp + 3.8 kb B. BamHI 800bp+ 3.2 kb C. Hindll+EcoRI :~1kb +~3kb D. Xhol+BamHI :~200bp+~800bp +~3kb Which one of the above digestion profiles confims successful cloning of the fragment in the vector in an orientation wherein the 5' end of the cloned fragment is towards P°? (1) A only (2) B only (3) A and C (4) C and D Options 1,. 1 2. 2 3. 3 4. 4
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  • Krishan k jakhad thankyou
    2 ...B only is correct option..since BamH1 site is also present in multiple cloning site. so if fragment is 5'-3' cloned..and cleaved with BamH1 so will generate 800 kb 3' fragment...
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