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Krishan k jakhad Best Answer
Medium specifically designed for human IVF was human tubal fluid (HTF), supplemented with either whole serum or with serum albumin, gained great popularity for the use of day 2 or day 3 human embryo cultures. there are no significant differences in statistical analysis in both media but single culture approach is advantageous in many different ways. 1. laboratory practices: required less Embryo handling, and thus minimum chances of unintentionally damaging the embryo. 2. adequate stabilization of embryo culture environment. therefore single media is growing in popularity.okay