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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Abhilasha Ganesh Tak posted an Question
April 14, 2021 • 11:54 am
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30 points
30 points
- CSIR NET
- Life Sciences
In fish the probe is constructed right, to localize the special dna sequences. are the probes of one or many types like have same sequence or different?
in fish the probe is constructed right, to localize the special DNA sequences. are the probes of one or many types like have same sequence or different?
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Krishan k jakhad![best-answer]()
at one time only one specific type of probe is used which is having same sequences. it will localize piece of DNA within genome or over chromosome. method for localizing a piece of DNA within a genome is called fluorescence in situ hybridization, abbreviated FISH. In this approach, a fluorescent dye is attached to a purified piece of DNA, and then that DNA is incubated with the full set of chromosomes from the originating genome, which have been attached to a glass microscope slide. The fluorescently labeled DNA finds its matching segment on one of the chromosomes, where it sticks. By looking at the chromosomes under a microscope, a researcher can find the region where the DNA is bound because of the fluorescent dye attached to it. This information thus reveals the location of that piece of DNA in the starting genome.