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In order to detect minor variations in antigen concentration, the following procedures were suggested. Which one will likely be the best option ?
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Eduncle Best Answer
To detect minor variations in antigen out of the options given competitive ELISA can be performed. In which the primary antibody (unlabeled) is incubated with sample antigen. Antibody-antigen complexes are then added to 96-well plates which are precoated with the same antigen. Unbound antibody is removed by washing the plate. (The more antigen in the sample, the less antibody will be able to bind to the antigen in the well, hence "competition.") The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The strength of the signal inversely proportional to antigen concentration.