Abhilasha Ganesh Tak posted an Question
April 06, 2021 • 11:12 am 30 points
  • CSIR NET
  • Life Sciences

In protein sequencing why the protein s are cleaved and again arranged as they were?

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    Krishan k jakhad best-answer

    Peptides longer than about 50-70 amino acids long cannot be sequenced reliably by the Edman degradation. Because of this, long protein chains need to be broken up into small fragments that can then be sequenced individually. strategy called divide and conquer successfully cleaves the larger protein into smaller and when sequenced we can find the adjoining fragments to find/predict our original large protein.

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    why the proteins are cleaved and rejoined again what is output of that?

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    already explained beta ji... proteins are large polymers so u need to break them into smaller fragments using peptidase enzymes right. output is protein sequencing. you will be able to know complete protein sequences of amino acids is it not enough 😀😎

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