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A. kirti Asked a Question
October 20, 2020 7:00 pmpts 30 pts
  • CSIR NET
  • Life Sciences

In southern blotting , after imprinting the dna pattern on nitrocellulose membrane how can we make ddna into sdna? is it done before imprinting or after imprint

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  • Anup padwal Best Answer
    It is done in denaturation step. which is the initial step of southern blot. it is done by heating the solution of dsDNA in to ssDNA by breaking H2 bond between them.
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