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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Abhilasha Ganesh Tak posted an Question
April 14, 2021 • 11:59 am
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30 points
30 points
- CSIR NET
- Life Sciences
Please explain the working of mc fish
please explain the working of Mc FISH in short inflow chart
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Krishan k jakhad![best-answer]()
multi-color (mc-FISH) is a ...special type, besides GISH Genomic in situ hybridization (GISH) and multicolor FISH (mc-FISH) represent two special types of FISH techniques. Both GISH and mc-FISH experiments have general steps and features of FISH, including chromosome preparation, probe labeling, blocking DNA preparation, target-probe DNA hybridization, post-hybridization washes, and hybridization signal detection. The mc-FISH takes advantage of simultaneous hybridization of several DNA probes labeled by different fluorochromes to different targets on the same chromosome sample. Hybridization signals from different probes are detected using different fluorescence filter sets. Multicolor FISH can provide more structural details for target chromosomes than single-color FISH obviously.
Multicolor FISH (M-FISH) assays are used for a precise assessment of complex chromosomal rearrangements. This technique uses all whole-chromosome painting probes in multiplex-FISH and spectral karyotyping. Thus, marker chromosomes, complex chromosomal rearrangements, and all numerical aberrations can be visualized simultaneously in a single hybridization experiment.