Simran Babbar Asked a Question
June 12, 2021 4:12 pmpts 30 pts
Proofreading E. coli RNA polymerase synthesizes RNA with remarkable fidelity in vivo. Its low error rate may be achieved, in part, by a proofreading mechanism found in DNA polyrmerases similar to that found in DNA polymerase proofreading involves two key events. The first event is a short backtracking motion of the enzyme along the DNA template through several base pairs. The movement is directed upstream in the opposite direction to transcriptional elongation (3' 5). DNA Cleaved3 RNA Elongating RNAP Back-tracted RNAP (S RNA The second event is nucleolytic cleavage, which occurs after a variable "pause" of the polymerase his delay can last anywhere from 20 seconds to 30 minutes in vitro. In its backtracked state, the olymerase can cleave off and discard the most recently added base(s) by nuclease activity. In this Ocess, a new 3 end is generated at the active site, ready for subsequent polymerization onto the ascent RNA chain.
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  • Simran Babbar
    what is actually backtracking?
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  • Krishan k jakhad thankyou
    RNA polymerase (abbreviated RNAP or RNApol, and officially DNA-directed (dependent) RNA polymerase), is an enzyme that synthesizes RNA from a DNA template. Unlike the proofreading...
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    Krishan k jakhad
    the mechanism is different is not the same as given in eduncle text that is a typing mistake alright. . In the RNA polymerase this occurs at the same active site used for po...
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  • Simran Babbar
    pls explain this proofreading part of transcription
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