Record the observation and result and write a brief discussion, results for mitosis with onion root tip using acetocarmine stain 

Priya sarda

Procedure Of The Experiment Place an onion on a tile With the help of a sharp blade, carefully snip the dry roots of the onion Place the bulbs in a beaker containing water to grow the root tips It may take around 4 to 6 days for the new roots to grow and appear Trim around 3 cm of the newly grown roots and place them in a watch glass With the help of forceps, shift it to a vial holding freshly prepared aceto-alcohol i.e., a mixture of glacial acetic acid and ethanol in the ratio 1:3 Allow the root tips to remain in the vial for one complete day With the help of forceps, pick one root and set in on a new glass slide With the help of a dropper, allow one drop of N/10 HCl to come in contact with the tip of the root. Additionally, add around 2 to 3 drops of the acetocarmine stain Heat it lightly on the burner in such a way that the stain does not dry up Excessive stain can be carefully treated using filter paper The more stained part of the root tip can be trimmed with the help of a blade. Discard the lesser stained part while retaining the more stained section Add a droplet of water to it With the help of a needle, a coverslip can be mounted on it Gently tap the coverslip with an unsharpened end of a needle in order for the meristematic tissue of the root tip present under the coverslip to be squashed properly and to be straightened out as a fine cell layer The onion root tip cells’ slide is now prepared and ready to be examined for different stages of mitosis Observe and study mitosis by placing the slide under the compound microscope. Focus as desired to obtain a distinct and clear image. Observations and Conclusion The slide containing the stained root tip cells is placed on the stage of the compound microscope, changes taking place are noted and sketched. The different phases of mitosis, such as prophase, metaphase, anaphase and telophase can be observed.