Sub-cellular fractionation-based assays have been used to identify various organelles in the mammalian cells. In order to characterize such organelles in a living mammalian cell, which of the following microscopy-base method would be the most accurate ?

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Ause of fluorescent probes specific for organelles.

Buse of organelle specific fluorescent probes followed by microinjection of fluorescent antibodies against organelle-specific protein.

Cuse of fluorescent probes in permeabilized cells.

Duse of organelle specific fluorescent probes followed by cryo-electron microscopy.

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Both of these fluorescent labels have different binding sites e.g. that respond to changes in microenvironments (e.g., pH, viscosity, and polarity) or quantities of biomolecules...
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