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To characterize the mechanism/s by which heat-stress is perceived in Arabidopsis., a team of researchers fused a Heat Shock promoter with luciferase gene. Transgenic plants having promoter luciferase fusion were raised. Such plants revealed strong luciferase expression upon heat-stress but they showed no expression under unstressed control condition. Subsequently, these transgenic plants were mutagenized by EMS and seeds from F2 generation were obtained. To analyze the downstream positive regulators of heat-stress, the researcher should analyze seedlings that are :
The question is about the perceiving of heat stress by Arabidopsis. So obviously under heat stress conditions, the heat stress promoter should be recognized and that leads to st...