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To characterize the mechanism/s by which heat-stress is perceived in Arabidopsis., a team of researchers fused a Heat Shock promoter with luciferase gene. Transgenic plants having promoter luciferase fusion were raised. Such plants revealed strong luciferase expression upon heat-stress but they showed no expression under unstressed control condition. Subsequently, these transgenic plants were mutagenized by EMS and seeds from F2 generation were obtained. To analyze the downstream positive regulators of heat-stress, the researcher should analyze seedlings that are :
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Eduncle Best Answer
The question is about the perceiving of heat stress by Arabidopsis. So obviously under heat stress conditions, the heat stress promoter should be recognized and that leads to strong expression of luciferase (which is the same as option 1, so these seedlings cannot be selected to analyze any downstream expression regulation). And control, does not express luciferase as the promoter has not been recognized as stress conditions are not there (again same condition as Option 2)
So now, the EMS mutagenesis, might mutate the heat stress promoter which renders it not capable of perceiving any heat stress conditions. In this case, if still luciferase is expressed means the expression is clearly controlled by some downstream positive regulators triggered by stress conditions justifying option 3 to be correct.
On the other hand, EMS mutating the luciferase enzyme, will not express luciferase in any conditions, so any kind of expressional regulation cannot be analyzed in seedlings that are not expressing luciferase even in stress conditions. So option 2 cannot be correct.