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Deepika sharma Asked a Question December 4, 2021 5:12 pm
20 pts unsuuCun 135 cTCCAG) and Smal (CCCIGGG), which one of the following combinations of restriction enzyme can neriment designed t0 clone a PCR-amplified fragment in a cloning vector digested with Xhol [CSIR NET JUNE 20171 Sed in the PCR primer to generate compatible ends for cloning?( indicates the site of cleavage within the recognition sequence)
a) Xbal (T/CTAGA) and Spel (A/CTAGT)
b) EcoRl (G/AATTc) and Smal(CCC/GGG)
c) Sall(G/TCGAC) and EcoRV (GAT/ATC)
d) Hindlll (A/AGCTT) and Pvull (CAG/CTG)
CSIR NET JUNE 2017
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