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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Priya sarda
Applications of ELISA The applications of ELISA are discussed below: The presence of antibodies and antigens in a sample can be determined. It is used in the food industry to detect any food allergens present. To determine the concentration of serum antibody in a virus test. During a disease outbreak, to evaluate the spread of the disease, e.g. during recent COVID-19 outbreak, rapid testing kits are being used to determine presence of antibodies in the blood sample. Direct ELISA (enzyme-linked immunosorbent assay) is a plate-based immunosorbent assay intended for the detection and quantification of a specific analyte (e.g. antigens, antibodies, proteins, hormones, peptides, etc.) from within a complex biological sample.
Ma'am I only need applications of 'DIRECT ELISA' not just ELISA
Applications of direct ELISA 1. Can be used to test specific antibody-to-antigen reactions 2. Can be used to eliminate cross-reactivity between other antibodies 3. It can be used for the detection and quantification of a specific analyte (e.g. antigens, antibodies, proteins, hormones, peptides, etc.) from within a complex biological sample. 4. It can be used when the immune response to an antigen needs to be analyzed