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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- CSIR NET
- Life Sciences
What is the role of detergent in this experiment ,dna isolation
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One can also use CTAB (Cetyl trimethyl ammonium bromide) to extract DNA. To the cell extract, CTAB is added which is a detergent like SDS. It helps in isolation of DNA with high purity by lysis of cell membrane of hepatocytes. In the plant cell, the concentration of the polysaccharides may be high which would precipitate with the DNA. CTAB would prevent the lipids, proteins and the polysaccharides from interacting with the DNA and coprecipitating with the DNA. The tube containing the sample is then centrifuged, and the complex would settle down in the form of a pellet. The protein, carbohydrate, lipids, etc. would be present in the supernatant and are discarded. The CTAB forms an insoluble complex with the DNA and settles down. The pellet is resuspended in the 1.5% NaCl. The CTAB and DNA would be separated, and on the addition of chilled ethanol, the DNA would be precipitated
CTAB (Cetyl trimethyl ammonium bromide) or SDS is used to break or dissolve cell membrane of hepatocytes and nuclear membrane so that DNA will come out in solution.