Zoo blot technique is similar to fish in what aspects

Krishan k jakhad

Yes both hybridization techniques like southern and can be used to compare the Genome of two biological species. zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different species. purpose is to detect the conservation of the gene(s) of interest throughout the evolution of different species. also can also help establish the locations of introns and exons, as exons are more conserved than introns. A zoo blot compares animal species while a garden blot compares plant species. In order to understand the degree to which a particular gene is similar from species to species, DNA extracts from a set of species are isolated and spread over a surface. Then, a gene probe added. Usually, the probe is marked with a radioactive isotope of phosphorus. Following the hybridization, autoradiography or other imaging techniques are used to identify successfully hybridized probes, proof of similarity between species' genomes. The hybridization between a probe and a segment of DNA will happen even when the strands are similar but not identical. As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. on the other hand FISH probes are often primers for the 16s rRNA region. FISH is widely used in the field of microbial ecology, to identify microorganisms. Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.