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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Arindam Paul posted an Question
December 17, 2020 • 13:55 pm
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
10. the ames test in a nutrient medium that lacks histi dine, a thin layer of agar containing 10 salmonella ty- phimurium histidine auxotrophs (mutant cells tha
10. The Ames Test In a nutrient medium that lacks histi dine, a thin layer of agar Containing 10 Salmonella ty- phimurium histidine auxotrophs (mutant cells that require histidine to survive) produces ~13 colonies over a twO-day incubation period at 37 °C (see Fig. 25-19). How do these colonies arise in the absence of histidine? The experimernt is repeated in the presence of 0.4 ug of 2-aminoanthracene. The number of colonies produced over two days exceeds 10,000. What does this indicate about 2-aminoanthracene? What can you surmise about its carcinogenicity? lant oolle
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Anup Padwal![best-answer]()
Answer 1: The presence of 13 colonies from 10^9 inoculated cells can be explained by spontaneous mutations (natural revertants were generated that could again encode the enzyme needed for histidine synthesis). Answer 2: The same experiment with the presence of 0.4 micrograms of 2-aminoanthracene resulted in 10,000 colonies. 2-aminoanthracene mutated the bacterial gene back to the wild type (his positive), so visible colonies appeared on the medium. Because the agent 2-aminoanthracene is a mutagen, it is therefore a possible carcinogen in humans.