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Arindam paul Asked a Question
May 21, 2021 7:33 pmpts 30 pts
  • IIT JAM
  • Biotechnology (BT)

12. if 5-termini of a linear dna molecule with sticky ends are being modified in a way that the ends cannot be ligated with each other anymore (to form a circul

12. If 5-termini of a linear DNA molecule with sticky ends are being modified in a way that the ends cannot be ligated with each other anymore (to form a circular molecule), which enzyme was used to modify the molecule? a. Methylase b. Kinase C. Phosphatase d. Dehydrogenase
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  • Abhijeet Gaurav Best Answer
    Correct answer is option ( C). Phosphatase enzyme Will remove the Phosphate group from the end of the terminal. Thus the -OH group won't be able to Form bond with next nucleotide ...
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