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Arindam Paul posted an Question
May 22, 2021 • 01:03 am
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
12. if 5-termini of a linear dna molecule with sticky ends are being modified in a way that the ends cannot be ligated with each other anymore (to form a circul
12. If 5-termini of a linear DNA molecule with sticky ends are being modified in a way that the ends cannot be ligated with each other anymore (to form a circular molecule), which enzyme was used to modify the molecule? a. Methylase b. Kinase C. Phosphatase d. Dehydrogenase
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Abhijeet Gaurav Best Answer
Correct answer is option ( C). Phosphatase enzyme Will remove the Phosphate group from the end of the terminal. Thus the -OH group won't be able to Form bond with next nucleotide molecule. Thus it wont be ligated each other again towards the ends. A phosphatase is an enzyme that catalyzes the hydrolysis of a nucleotide, forming a nucleoside and a phosphate ion.