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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Anam Fatma posted an Question
November 23, 2021 • 17:44 pm
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20 points
20 points
- IIT JAM
- Biotechnology (BT)
2. pure igg antibody was run on an sds-page under reducing condition. how many bands would you see after staining with coomassie blue? (1) 4 (2) 2 (3) 1 (4) 6
2. Pure IgG antibody was run on an SDS-PAGE under reducing condition. How many bands would you see after staining with Coomassie blue? (1) 4 (2) 2 (3) 1 (4) 6
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Sanjana gupta
Purifying a monoclonal antibody from a hybridoma solution, and when we run it on an SDS-PAGE gel I get 2 bands for the light chain. -we know that an antibody is 150 KDa and when we boil it in the presence of DTT and run it on an SDS-PAGE gel we should get 2 bands, one for the heavy chain at 50 KDa and the other one for the light chain at 25 KDa (An antibody consists of 2 heavy chains and 2 light chains). -Though we can get 2 bands for the heavy chain (as it can get glycosylated)
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sorry but answer is 1 band
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could you please explain it...for better understanding
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