Anam Fatma posted an Question
November 23, 2021 • 17:44 pm 20 points
  • IIT JAM
  • Biotechnology (BT)

2. pure igg antibody was run on an sds-page under reducing condition. how many bands would you see after staining with coomassie blue? (1) 4 (2) 2 (3) 1 (4) 6

2. Pure IgG antibody was run on an SDS-PAGE under reducing condition. How many bands would you see after staining with Coomassie blue? (1) 4 (2) 2 (3) 1 (4) 6

1 Answer(s) Answer Now
  • 0 Likes
  • 8 Comments
  • 0 Shares
  • Sanjana gupta

    Purifying a monoclonal antibody from a hybridoma solution, and when we run it on an SDS-PAGE gel I get 2 bands for the light chain.  -we know that an antibody is 150 KDa and when we boil it in the presence of DTT and run it on an SDS-PAGE gel we should get 2 bands, one for the heavy chain at 50 KDa and the other one for the light chain at 25 KDa (An antibody consists of 2 heavy chains and 2 light chains). -Though we can get 2 bands for the heavy chain (as it can get glycosylated)

    eduncle-logo-app

    sorry but answer is 1 band

    eduncle-logo-app

    could you please explain it...for better understanding

whatsapp-btn

Do You Want Better RANK in Your Exam?

Start Your Preparations with Eduncle’s FREE Study Material

  • Updated Syllabus, Paper Pattern & Full Exam Details
  • Sample Theory of Most Important Topic
  • Model Test Paper with Detailed Solutions
  • Last 5 Years Question Papers & Answers