Anam fatma Asked a Question
November 23, 2021 12:14 pmpts 20 pts
2. Pure IgG antibody was run on an SDS-PAGE under reducing condition. How many bands would you see after staining with Coomassie blue? (1) 4 (2) 2 (3) 1 (4) 6
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  • Sanjana gupta
    Purifying a monoclonal antibody from a hybridoma solution, and when we run it on an SDS-PAGE gel I get 2 bands for the light chain.  -we know that an antibody is 150 KDa and when ...
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    Anam fatma
    sorry but answer is 1 band
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