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Arindam Paul posted an Question
May 22, 2021 • 02:38 am
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
.23 which one of the following microscopic techniques provides a 3-dimensional perspective of live. unstained and transparent specimens obtained from the wild?
.23 Which one of the following microscopic techniques provides a 3-dimensional perspective of live. unstained and transparent specimens obtained from the wild? stions 1. Fluorescence microscopy Differential interference contrast (Nomarski) 2. microscopy 3. Confocal microscopy 4. Phase contrast microscopy
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Abhijeet Gaurav Best Answer
Correct answer is option (B). Differential Interference Contrast (DIC) is used for imaging live and unstained biological samples, such as a smear from a tissue culture or individual water borne single-celled organisms. Its resolution and clarity in conditions such as this are unrivaled among standard optical microscopy techniques. Image quality, when used under suitable conditions, is outstanding in resolution and almost entirely free of artifacts unlike phase contrast.