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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
8. an mrna coding for a secretory protein, when translated using free ribosome under in vitro conditions, resulted in a 40 kda protein. the same mrna when trans
8. An mRNA cOding for a secretory protein, when translated using free riboSome under in vitro conditions, resulted in a 40 kDa protein. The same mRNA when translated using the rough endoplasmic reticulum resulted in a 36 kDa protein. The difference in the molecular weight of the two polypeptides is due to the loss of a (a) 2 kDa peptide from N-terminus and a 2 kDa peptide from the C-terminus kDa peptide from the C-terminus 4 kDa peptide from the C-terminus (b) 1 kDa peptide from N-terminus and a 3 (c) 4 kDa peptide from the N-terminuss (d)
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Narayan singh Best Answer
After being synthesized with the Rough Endoplasmic Reticulum, the protein gets translocated to the interior lumen of the RER with the help of a signaling peptide. This signaling peptide gets cleaved later on thus producing a truncated functional protein. The signaling peptide usually lies on the N-terminal of the protein. So the correct option is '4 kDa peptide from the N-terminus'.