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Arindam Paul posted an Question
August 31, 2020 • 21:52 pm
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
A 0.1% (w/v) solution of a protein absorbs 20% of the incident light. what fraction of light is transmitted if the concentration is increased to 0.4%? [
A 0.1% (w/v) solution of a protein absorbs 20% of the incident light. What fraction of light is transmitted if the concentration is increased to 0.4%? [
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Anup Padwal
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The absorbance is directly proportional to the concentration of the solution. Some light is absorbed as it passes through a solution so less light is transmitted. Doubling the concentration doubles the absorbance. Doubling the path length doubles the absorbance. here the pathlenght is constant. The concentration is increased to 4 times so it will absorb 4 times of the light. To understand this concenpt remind the protein estimation assay performed in BSc. In which the series of standards is prepared first tube of standard absorb half of the light than the second tube a d so on. Also refer Lambert-Beer's law.
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Priyanshu kumar Best Answer
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