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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
Gauri Sharma posted an Question
September 02, 2022 • 17:16 pm
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
A double stranded dna molecule of total 5000 base pairs long, has a melting temperature of 85'c. what will be the % at base pairs in this sample? (up to one dec
Please explain me the detailed solution of this question
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Anupriya bandyopadhyay![best-answer]()
More the no. of GC, more will be the Tm (melting temperature)..... since, G and C have 3 H bonds, while A and T have 2 H bonds. There is a formula to find the Tm for these kind of sums where you have to correlate % of bp with Tm. The formula is- Tm= 64.9+ 0.41 (%GC) - (500/ length of DNA) [NOTE- This formula comes from significant experimental evidences for relating Tm to DNA hybridization. The deviation involves lots of exponential and logarithmic functions which you will not need for any exam. Just remember the above formula for Tm vs % GC] For this sum, Tm= 64.9 + 0.41(%GC)- (500/5000) 85= 64.9+ 0.41 (%GC) - 0.1 20.2= 0.41 (%GC) %GC= 49.26 Hence, % AT= 100- 49.26 = - 50.73 Hope the answer helps
Please say what clarification is required? As said the formula comes from complex calculations and wouldn't have time to do such mathematics in the exam hall.....so try to remember the formula......Tm= 64.9 + 0.41(%GC)- (500/ length)