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A new penicillium mutant was tested for auxotrophy. the mutant grows on minimal medium (m) +arginine (a) + proline (p) + histidine (h) but not on m or on m +a+h
A new Penicillium mutant was tested for auxotrophy. The mutant grows on minimal medium (M) +arginine (A) + proline (P) + histidine (H) but not on M or on M +A+H. The mutant requires
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Abhijeet Gaurav Best Answer
It is an Proline Auxotroph Mutant. It requires Proline to grow. The non-naturally occurring proline auxotroph, wherein the proline auxotroph is a methanotrophic microorganism comprising an altered endogenous proC gene, wherein the proline auxotroph exhibits a growth phenotype of no growth when cultured in a proline-free culture medium and in the presence of a C1 substrate. The non-naturally occurring proline auxotroph of claim 1, wherein the proline auxotroph exhibits a growth phenotype of growth when cultured in a proline-containing medium, the proline-containing culture medium comprising from about µg/mL to about 500 µg/mL of proline.