A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain ofe. coli having the genotype lacl crp". even in the abs

A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain ofE. coli having the genotype lacl crp". Even in the absence of inducer IPTG. low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression? (A) Addition of lactose to the medium (B) Removal of all glucose from the medium (C) Addition of excess glucose to the mediunm (D) Addition of allo-lactose to the medium