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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain ofe. coli having the genotype lacl crp". even in the abs
A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain ofE. coli having the genotype lacl crp". Even in the absence of inducer IPTG. low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression? (A) Addition of lactose to the medium (B) Removal of all glucose from the medium (C) Addition of excess glucose to the mediunm (D) Addition of allo-lactose to the medium
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Dr. sharad agrawal![best-answer]()
option c, addition of excess glucose in the medium bcoz if glucose is present in excess, bacteria would prefer to utilize glucose over lactose due to which the lac operon model will remain switch off. more glucose results in more ATP and less cAMP due to which cAMP CAP protein complex is not formed and lac operon is further repressed. this effect is also known as the glucose effect.