Swastika Asked a Question
December 24, 2021 7:13 ampts 30 pts
An enzyme requires both aspartate (pKa of side chain = 4.5) and histidine (pKa of side chain = 6.5) residues in the catalytic site to be protonated for activity. The expected enzyme activity (in %) at a pH of 5.5 would be closest to A 90 B. 78 C. 50 D. 10 all'c
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  • Swastika
    in the answer key its showing that option D is correct
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  • Priya sarda thankyou
    90% PKa=4.5 and PKb=6.5 Therefore, PI = 4.5+6.5÷2=5.5 The optimum pH depends on the amino acid components of active site. The Isoelectric point depends of the full enzyme primary a...
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