IIT JAM
Nisarg Asked a Question
May 31, 2021 4:34 pmpts 30 pts
  • IIT JAM
  • Biotechnology (BT)

As we know most of the endonucleases only cut at specific dsdna site having palindrome, and also we know that palindrome is only possible when dsdna is present

As we know most of the endonucleases only cut at specific dsDNA site having palindrome, and also we know that palindrome is only possible when dsDNA is present my doubt is that what about those endonucleases that are specific for ssDNA or RNA like S1nuclease how will they identify the specific region to cut because here palindrome is not possible?
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  • Abhijeet Gaurav Best Answer
    ssDNA has it's own specific endonuclease site but it's not palindrome Sequence like dsDNA. It usually have poly G or poly A like sequence that is being recognised & thus they cut...
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