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As we know most of the endonucleases only cut at specific dsdna site having palindrome, and also we know that palindrome is only possible when dsdna is present
As we know most of the endonucleases only cut at specific dsDNA site having palindrome, and also we know that palindrome is only possible when dsDNA is present my doubt is that what about those endonucleases that are specific for ssDNA or RNA like S1nuclease how will they identify the specific region to cut because here palindrome is not possible?
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Abhijeet Gaurav Best Answer
ssDNA has it's own specific endonuclease site but it's not palindrome Sequence like dsDNA. It usually have poly G or poly A like sequence that is being recognised & thus they cut at such sites. The known RNA endonucleases either cleave their targets through recognition of specific structures (for example, RNase III family or RNase H) or have limited sequence specificity (for example, RNase A or RNase T1). There exist DNA/RNA non-specific endonucleases, such as those that are found in Serratia marcescens, which act on dsDNA, ssDNA, and RNA.