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Arindam Paul posted an Question
January 19, 2021 • 22:53 pm
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30 points
30 points
- IIT JAM
- Biotechnology (BT)
Choose the correct statement(s) about thermostable dna polymerase used in pcr q. taq pol has 5 3 exonuclease activity. r. pfu pol has 3 5 exonuclease activity.
Choose the correct statement(s) about thermostable DNA polymerase used in PCR Q. Taq Pol has 5 3 exonuclease activity. R. Pfu Pol has 3 5 exonuclease activity. S. Tli Pol has no 3 5 exonuclease activity. P. Taq Pol has no proofreading ability. a. P and Q b. P, Q and R ons C. 2, R and s d. P, Q, R and S
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Yogita Basnal Best Answer
option b is correct i.e. statements P, Q and R are correct. Taq polymerase has 5'-3' exonuclease activity so statement 'Q' is correct. Taq polymerase lacks proof reading activity so it very fast, it can add 1000 bases in a minute so statement 'P' is correct Pfu has 3'-5' activity which means that it has proofreading activity which makes it slow. It can add 500 bases in a minute. Based on this statement 'R' is correct. Tli polymerase has proof reading activityactivity i. e. 3' to 5' exonuclease activity. So statement 'S' is incorrect.