Mohanchaitanya reddy Asked a Question
April 28, 2020 2:58 pmpts 30 pts
Enzymatic cleavage: trypsin is most commonly used. It hydrolyses the peptide bonds containing lysine or arginine on the carbonyl (-C=0) side of peptide linkage. Chemical cleavage: Cyanogen bromide (CNBr) is commonly used to split polypeptides into smaller fragments. CNBr specifically splits peptide bonds, the carbonyl side of which is contributed by the amino acid methionine. please explain diagrammatically
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  • Abhijeet Gaurav thankyou
    The have common type of mechanism with all 3 Serine Proteases & there is no specific mechanism anywhere in either Lehninger or Voet & Voet.
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  • Abhijeet Gaurav Best Answer
    Cyanogen bromide hydrolyzes peptide bonds at the C-terminus of methionine residues. This reaction is used to reduce the size of polypeptide segments for identification and sequenci...
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    Mohanchaitanya reddy
    but it was mentioned that trypsin will hydrolyze at aspergine on carbonyl side of petide bond