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Nilanjan Bhowmick AIR 3, CSIR NET (Earth Science)
- IIT JAM
- Biotechnology (BT)
Estimate the net charge of a peptide ser-glu-pro-ile-met-ala-pro-val-glu-tyr-pro-lys at ph 7.0 and at ph 12.0. typical ranges observed for pk, values of groups
Estimate the net charge of a peptide Ser-Glu-Pro-Ile-Met-Ala-Pro-Val-Glu-Tyr-Pro-Lys at pH 7.0 and at pH 12.0. Typical ranges observed for pK, values of groups in protein: a-carboxyl group (3.5-4.0), side chain carboxyl group (4.0-4.8), a-amino group (8.0-9.0), phenolic group (9.5-10.5), side chain amino group (9.8-10.4). a.-1,-4 C.-4, -4 31. b.-4, -1 d. -1, -1 [IIT-JAM] Tho biab colubilitc
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Mohanchaitanya reddy![best-answer]()
first you have to start from a completely protoanated form of the peptide and then you have to remove the proton(H+) based on the given pH value, then calculate the overall charge on the peptide at certain pH. I am attaching a picture , I hope that it might help you with your question.